Composite

Part:BBa_K4494196

Designed by: Shiran Yuan   Group: iGEM22_DKU_China   (2022-09-30)


Promoter-RBS Combination for A. caldus csoR

This part consists of PIII (K4494002) and an RBS (B0034) combined using Golden Gate Assembly and works with A. caldus csoR (K4494001). Its variants are parts K4494003-K4494098.

Note: This part does NOT work. For a working part please check our collection of variants K4494003-K4494098.

The K4494003-K4494098 Mutant Collection

Parts K4494003-K4494098 are mutants of K4494196 generated and characterized via high-throughput experimentation. For more information regarding this collection please check out our wiki.The following shows some important statistical data regarding the collection.

Characterization

Heatmap Characterization

Phylogeny

The following is the minimum-evolution tree of our collection (the numbers are variant order codes):

Mimimum-Evolution Tree

Scatter Plot

The following scatter plot encompasses the foldchange, blank RFU, and Levenshtein Distance to K4494196:

Scatter Plot

Base Pair Count

Unweighted Base Pair Frequency by Position
Unweighted Base Pair Percentages by Position

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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